The use of filter paper discs impregnated with thionin acetate, basic fuchsin and thionin blue in the identification of Brucella species.

Filter paper discs impregnated with solutions containing 0.25, 0.5 and 1 mg/milliliter of thionin acetate, 0.75 and 1.5 mg/milliliter of basic fuchsin and 0.5 mg/milliliter of thionin blue were used in the typing of Brucella species. All the strains used reacted as expected, proving this new technique to be reliable in the identification of Brucella species. The method is less expensive and the results easier to interpret than those obtained with methods previously used.